Synergistic and tunable human gene activation by combinations of synthetic transcription factors.

TitleSynergistic and tunable human gene activation by combinations of synthetic transcription factors.
Publication TypeJournal Article
Year of Publication2013
AuthorsP Perez-Pinera, DG Ousterout, JM Brunger, AM Farin, KA Glass, F Guilak, GE Crawford, AJ Hartemink, and CA Gersbach
JournalNat Methods
Volume10
Start Page239
Issue3
Pagination239 - 242
Date Published03/2013
Abstract

Mammalian genes are regulated by the cooperative and synergistic actions of many transcription factors. In this study we recapitulate this complex regulation in human cells by targeting endogenous gene promoters, including regions of closed chromatin upstream of silenced genes, with combinations of engineered transcription activator-like effectors (TALEs). These combinations of TALE transcription factors induced substantial gene activation and allowed tuning of gene expression levels that will broadly enable synthetic biology, gene therapy and biotechnology.

DOI10.1038/nmeth.2361
Short TitleNat Methods