Runx2-stimulated transdifferentiation of primary skeletal myoblasts into an osteoblastic mineralizing phenotype for bone tissue engineering

TitleRunx2-stimulated transdifferentiation of primary skeletal myoblasts into an osteoblastic mineralizing phenotype for bone tissue engineering
Publication TypeJournal Article
Year of Publication2004
AuthorsCA Gersbach, BA Byers, RE Guldberg, GK Pavlath, and AJ García
JournalTransactions - 7th World Biomaterials Congress
Start Page328-
Date Published01/2004
Abstract

The Runx2-stimulated transdifferentiation in skeletal primary myoblasts into an osteoblastic phenotype for bone tissue engineering, was investigated. The primary myoblasts were isolated from Balb/c mice and cultured in growth media. The microscopy and immunofluorescent staining exhibited reduced myotube fusion in Runx2-transduced myoblasts, which suggested inhibited myogenesis. The scaffolds seeded with Runx2-overexpressing myoblasts displayed regions of high X-ray attenuation by microCT and elevated elastic moduli by compression analysis at 42 days. The results show that the forced expression of Runx2 may bypass parallel BMP-2 stimulated regulatory pathways, leading to increased osteogenesis and mineralization.

Short TitleTransactions - 7th World Biomaterials Congress