Title | Runx2-stimulated transdifferentiation of primary skeletal myoblasts into an osteoblastic mineralizing phenotype for bone tissue engineering |
Publication Type | Journal Article |
Year of Publication | 2004 |
Authors | CA Gersbach, BA Byers, RE Guldberg, GK Pavlath, and AJ García |
Journal | Transactions - 7th World Biomaterials Congress |
Start Page | 328 |
Date Published | 12/2004 |
Abstract | The Runx2-stimulated transdifferentiation in skeletal primary myoblasts into an osteoblastic phenotype for bone tissue engineering, was investigated. The primary myoblasts were isolated from Balb/c mice and cultured in growth media. The microscopy and immunofluorescent staining exhibited reduced myotube fusion in Runx2-transduced myoblasts, which suggested inhibited myogenesis. The scaffolds seeded with Runx2-overexpressing myoblasts displayed regions of high X-ray attenuation by microCT and elevated elastic moduli by compression analysis at 42 days. The results show that the forced expression of Runx2 may bypass parallel BMP-2 stimulated regulatory pathways, leading to increased osteogenesis and mineralization. |
Short Title | Transactions - 7th World Biomaterials Congress |