RNA-guided gene activation by CRISPR-Cas9-based transcription factors

TitleRNA-guided gene activation by CRISPR-Cas9-based transcription factors
Publication TypeJournal Article
Year of Publication2013
AuthorsP Perez-Pinera, DD Kocak, CM Vockley, AF Adler, AM Kabadi, LR Polstein, PI Thakore, KA Glass, DG Ousterout, KW Leong, F Guilak, GE Crawford, TE Reddy, and CA Gersbach
JournalNature Methods
Volume10
Start Page973
Issue10
Pagination973 - 976
Date Published01/2013
Abstract

Technologies for engineering synthetic transcription factors have enabled many advances in medical and scientific research. In contrast to existing methods based on engineering of DNA-binding proteins, we created a Cas9-based transactivator that is targeted to DNA sequences by guide RNA molecules. Coexpression of this transactivator and combinations of guide RNAs in human cells induced specific expression of endogenous target genes, demonstrating a simple and versatile approach for RNA-guided gene activation.

DOI10.1038/nmeth.2600
Short TitleNature Methods