Title | Light-inducible gene regulation with engineered zinc finger proteins. |
Publication Type | Journal Article |
Year of Publication | 2014 |
Authors | LR Polstein, and CA Gersbach |
Journal | Methods in molecular biology (Clifton, N.J.) |
Volume | 1148 |
Start Page | 89 |
Pagination | 89 - 107 |
Date Published | 01/2014 |
Abstract | The coupling of light-inducible protein-protein interactions with gene regulation systems has enabled the control of gene expression with light. In particular, heterodimer protein pairs from plants can be used to engineer a gene regulation system in mammalian cells that is reversible, repeatable, tunable, controllable in a spatiotemporal manner, and targetable to any DNA sequence. This system, Light-Inducible Transcription using Engineered Zinc finger proteins (LITEZ), is based on the blue light-induced interaction of GIGANTEA and the LOV domain of FKF1 that drives the localization of a transcriptional activator to the DNA-binding site of a highly customizable engineered zinc finger protein. This chapter provides methods for modifying LITEZ to target new DNA sequences, engineering a programmable LED array to illuminate cell cultures, and using the modified LITEZ system to achieve spatiotemporal control of transgene expression in mammalian cells. |
DOI | 10.1007/978-1-4939-0470-9_7 |
Short Title | Methods in molecular biology (Clifton, N.J.) |