| Title | A light-inducible CRISPR-Cas9 system for control of endogenous gene activation |
| Publication Type | Journal Article |
| Year of Publication | 2015 |
| Authors | LR Polstein, and CA Gersbach |
| Journal | Nature Chemical Biology |
| Volume | 11 |
| Issue | 3 |
| Start Page | 198 |
| Pagination | 198 - 200 |
| Date Published | 01/2015 |
| Abstract | Optogenetic systems enable precise spatial and temporal control of cell behavior. We engineered a light-activated CRISPR-Cas9 effector (LACE) system that induces transcription of endogenous genes in the presence of blue light. This was accomplished by fusing the light-inducible heterodimerizing proteins CRY2 and CIB1 to a transactivation domain and the catalytically inactive dCas9, respectively. The versatile LACE system can be easily directed to new DNA sequences for the dynamic regulation of endogenous genes. |
| DOI | 10.1038/nchembio.1753 |
| Short Title | Nature Chemical Biology |
