A light-inducible CRISPR-Cas9 system for control of endogenous gene activation.

TitleA light-inducible CRISPR-Cas9 system for control of endogenous gene activation.
Publication TypeJournal Article
Year of Publication2015
AuthorsPolstein, LR, and Gersbach, CA
JournalNature Chemical Biology
Volume11
Issue3
Start Page198
Pagination198 - 200
Date Published03/2015
Abstract

Optogenetic systems enable precise spatial and temporal control of cell behavior. We engineered a light-activated CRISPR-Cas9 effector (LACE) system that induces transcription of endogenous genes in the presence of blue light. This was accomplished by fusing the light-inducible heterodimerizing proteins CRY2 and CIB1 to a transactivation domain and the catalytically inactive dCas9, respectively. The versatile LACE system can be easily directed to new DNA sequences for the dynamic regulation of endogenous genes.

DOI10.1038/nchembio.1753
Short TitleNature Chemical Biology