|Title||A light-inducible CRISPR-Cas9 system for control of endogenous gene activation.|
|Publication Type||Journal Article|
|Year of Publication||2015|
|Authors||LR Polstein, and CA Gersbach|
|Journal||Nature Chemical Biology|
|Pagination||198 - 200|
Optogenetic systems enable precise spatial and temporal control of cell behavior. We engineered a light-activated CRISPR-Cas9 effector (LACE) system that induces transcription of endogenous genes in the presence of blue light. This was accomplished by fusing the light-inducible heterodimerizing proteins CRY2 and CIB1 to a transactivation domain and the catalytically inactive dCas9, respectively. The versatile LACE system can be easily directed to new DNA sequences for the dynamic regulation of endogenous genes.
|Short Title||Nature Chemical Biology|