Engineering synthetic TALE and CRISPR/Cas9 transcription factors for regulating gene expression

TitleEngineering synthetic TALE and CRISPR/Cas9 transcription factors for regulating gene expression
Publication TypeJournal Article
Year of Publication2014
AuthorsAM Kabadi, and CA Gersbach
JournalMethods
Volume69
Issue2
Start Page188
Pagination188 - 197
Date Published01/2014
Abstract

© 2014 Elsevier Inc. Engineered DNA-binding proteins that can be targeted to specific sites in the genome to manipulate gene expression have enabled many advances in biomedical research. This includes generating tools to study fundamental aspects of gene regulation and the development of a new class of gene therapies that alter the expression of endogenous genes. Designed transcription factors have entered clinical trials for the treatment of human diseases and others are in preclinical development. High-throughput and user-friendly platforms for designing synthetic DNA-binding proteins present innovative methods for deciphering cell biology and designing custom synthetic gene circuits. We review two platforms for designing synthetic transcription factors for manipulating gene expression: Transcription activator-like effectors (TALEs) and the RNA-guided clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 system. We present an overview of each technology and a guide for designing and assembling custom TALE- and CRISPR/Cas9-based transcription factors. We also discuss characteristics of each platform that are best suited for different applications.

DOI10.1016/j.ymeth.2014.06.014
Short TitleMethods