The Development of TALE Nucleases for Biotechnology.

TitleThe Development of TALE Nucleases for Biotechnology.
Publication TypeJournal Article
Year of Publication2016
AuthorsDG Ousterout, and CA Gersbach
JournalMethods in molecular biology (Clifton, N.J.)
Start Page27
Pagination27 - 42
Date Published01/2016

The development of a facile genome engineering technology based on transcription activator-like effector nucleases (TALENs) has led to significant advances in diverse areas of science and medicine. In this review, we provide a broad overview of the development of TALENs and the use of this technology in basic science, biotechnology, and biomedical applications. This includes the discovery of DNA recognition by TALEs, engineering new TALE proteins to diverse targets, general advances in nuclease-based editing strategies, and challenges that are specific to various applications of the TALEN technology. We review examples of applying TALENs for studying gene function and regulation, generating disease models, and developing gene therapies. The current status of genome editing and future directions for other uses of these technologies are also discussed.

Short TitleMethods in molecular biology (Clifton, N.J.)