CRISPR-based methods for high-throughput annotation of regulatory DNA.

TitleCRISPR-based methods for high-throughput annotation of regulatory DNA.
Publication TypeJournal Article
Year of Publication2018
AuthorsTS Klann, JB Black, and CA Gersbach
JournalCurrent Opinion in Biotechnology
Volume52
Start Page32
Pagination32 - 41
Date Published08/2018
Abstract

Developments in CRISPR/Cas9-based technologies provide a new paradigm in functional screening of the genome. Conventional screening methods have focused on high-throughput perturbations of the protein-coding genome with technologies such as RNAi. However, equivalent methods for perturbing the non-coding genome have not existed until recently. CRISPR-based screening of genomic DNA has enabled the study of both genes and non-coding gene regulatory elements. Here we review recent progress in assigning function to the non-coding genome using CRISPR-based genomic and epigenomic screens, and discuss the prospects of these technologies to transforming our understanding of genome structure and regulation.

DOI10.1016/j.copbio.2018.02.004
Short TitleCurrent Opinion in Biotechnology