Advances in targeted genome editing.

TitleAdvances in targeted genome editing.
Publication TypeJournal Article
Year of Publication2012
AuthorsPerez-Pinera, P, Ousterout, DG, and Gersbach, CA
JournalCurrent Opinion in Chemical Biology
Volume16
Issue3-4
Start Page268
Pagination268 - 277
Date Published08/2012
Abstract

New technologies have recently emerged that enable targeted editing of genomes in diverse systems. This includes precise manipulation of gene sequences in their natural chromosomal context and addition of transgenes to specific genomic loci. This progress has been facilitated by advances in engineering targeted nucleases with programmable, site-specific DNA-binding domains, including zinc finger proteins and transcription activator-like effectors (TALEs). Recent improvements have enhanced nuclease performance, accelerated nuclease assembly, and lowered the cost of genome editing. These advances are driving new approaches to many areas of biotechnology, including biopharmaceutical production, agriculture, creation of transgenic organisms and cell lines, and studies of genome structure, regulation, and function. Genome editing is also being investigated in preclinical and clinical gene therapies for many diseases.

DOI10.1016/j.cbpa.2012.06.007
Short TitleCurrent Opinion in Chemical Biology